MCP-1, also known as CCL2, is expressed by a range of cells including monocytes, vascular endothelial cells, smooth muscle cells, osteoblasts and glomeruler mesangial cells. MCP-1 plays an important role in the inflammatory response of blood monocytes and tissue macrophages. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunohistochemistry (Frozen/paraffin): 1:250-1:500 Immunocytochemistry: 1:1000 ELISA: Capture Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
