Retinol binding protein (RBP) is responsible for binding and transporting retinol (vitamin A). It has a binding site for one molecule of retinol and circulates in the plasma together with prealbumin in the form of a protein complex in a molar ratio of 1:1. This binding to prealbumin prevents greater glomerular losses of the human retinol-binding protein. Only the retinol-free form of the retinol-binding protein,which has no affinity for prealbumin, undergoes glomerular filtration unhindered as a result of its low molecular weight. Applications: Suitable for use in ELISA or for antigen applications in immunological protocols. Other applications not tested. Electrophoresis: One band with alpha2 electrophoretic mobility Protein: Reported in mg/vial (A280nm, E0.1%=1.94) Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
