MSR1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB100585
- Bilder (6)
| Artikelname: | MSR1 Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB100585 |
| Hersteller Artikelnummer: | orb100585 |
| Alternativnummer: | BYT-ORB100585-50,BYT-ORB100585-100,BYT-ORB100585-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | WB |
| Spezies Reaktivität: | Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human MSR1/CD204 (211-320/451aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | CD204, SCARA1, SR-A, SR-AI, SR-AII, SR-AIII, SRA, phSR1, phSR2, RGD1564316, MSRE_BOVIN, MSR1, Macrophage acetylated LDL receptor I and II, MSRE_HUMAN, Scavenger receptor class A member 1, |
| MSR1 Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 50 kDa |
| UniProt: | P21757 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | MSR1 |
| Application Verdünnung: | WB=1:500-2000 |
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Blank control (Blue): U937 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-MSR1 antibody (orb100585), Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions), Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. |
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Sample: Bone (Mouse) Lysate at 40 ug, Primary: Anti-MSR1 (orb100585) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD. |
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Sample: Hela Cell (Human) Lysate at 40 ug, Primary: Anti-MSR1 (orb100585) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD. |
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Sample: Lane 1: Spleen (Mouse) Lysate at 40 ug, Lane 2: Lung (Mouse) Lysate at 40 ug, Lane 3: Liver (Mouse) Lysate at 40 ug, Lane 4: Placenta (Mouse) Lysate at 40 ug, Lane 5: Cerebrum (Mouse) Lysate at 40 ug, Lane 6: Raw264.7 (Mouse) Cell Lysate at 30 ug, Lane 7: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 8: Liver (Rat) Lysate at 40 ug, Lane 9: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-MSR1 (orb100585) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50/39 kD, Observed band size: 50 kD. |
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Tissue/cell: human colon carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MSR1 Polyclonal Antibody, FITC conjugated 1:100, 60 minutes at 37C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei. |
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Tissue/cell: mouse testis tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MSR1 Polyclonal Antibody, FITC conjugated 1:100, 60 minutes at 37C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei. |
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