MSR1 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB100585
Article Name: MSR1 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB100585
Supplier Catalog Number: orb100585
Alternative Catalog Number: BYT-ORB100585-50,BYT-ORB100585-100,BYT-ORB100585-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: WB
Species Reactivity: Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human MSR1/CD204 (211-320/451aa)
Conjugation: Unconjugated
Alternative Names: CD204, SCARA1, SR-A, SR-AI, SR-AII, SR-AIII, SRA, phSR1, phSR2, RGD1564316, MSRE_BOVIN, MSR1, Macrophage acetylated LDL receptor I and II, MSRE_HUMAN, Scavenger receptor class A member 1,
MSR1 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 50 kDa
UniProt: P21757
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: MSR1
Application Dilute: WB=1:500-2000
Blank control (Blue): U937 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-MSR1 antibody (orb100585), Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions), Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Sample: Bone (Mouse) Lysate at 40 ug, Primary: Anti-MSR1 (orb100585) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD.
Sample: Hela Cell (Human) Lysate at 40 ug, Primary: Anti-MSR1 (orb100585) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD.
Sample: Lane 1: Spleen (Mouse) Lysate at 40 ug, Lane 2: Lung (Mouse) Lysate at 40 ug, Lane 3: Liver (Mouse) Lysate at 40 ug, Lane 4: Placenta (Mouse) Lysate at 40 ug, Lane 5: Cerebrum (Mouse) Lysate at 40 ug, Lane 6: Raw264.7 (Mouse) Cell Lysate at 30 ug, Lane 7: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 8: Liver (Rat) Lysate at 40 ug, Lane 9: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-MSR1 (orb100585) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50/39 kD, Observed band size: 50 kD.
Tissue/cell: human colon carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MSR1 Polyclonal Antibody, FITC conjugated 1:100, 60 minutes at 37C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.
Tissue/cell: mouse testis tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MSR1 Polyclonal Antibody, FITC conjugated 1:100, 60 minutes at 37C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.