UGDH Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB101134
- Bilder (6)
| Artikelname: | UGDH Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB101134 |
| Hersteller Artikelnummer: | orb101134 |
| Alternativnummer: | BYT-ORB101134-50,BYT-ORB101134-100,BYT-ORB101134-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human UGDH (421-494/494aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | DEE84, EIEE84, GDH, UDP-GlcDH, UDPGDH, UGD, UGDH_HUMAN, UGDH, UDP-Glc dehydrogenase, 1.1.1.22, UGDH_MOUSE, UGDH_RAT, |
| UGDH Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 55 kDa |
| UniProt: | O60701 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | UGDH |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded (human descend colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (UGDH) Polyclonal Antibody, Unconjugated (orb101134) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (UGDH) Polyclonal Antibody, Unconjugated (orb101134) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (UGDH) Polyclonal Antibody, Unconjugated (orb101134) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (UGDH) Polyclonal Antibody, Unconjugated (orb101134) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Large intestine (Mouse) Lysate at 40 ug, Primary: Anti-UGDH (orb101134) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kD, Observed band size: 55 kD. |
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Sample: Liver (Mouse) Lysate at 40 ug, Stomach (Mouse) Lysate at 40 ug, Primary: Anti-UGDH (orb101134) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kD, Observed band size: 55 kD. |
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