UGDH Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB101134
Article Name: UGDH Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB101134
Supplier Catalog Number: orb101134
Alternative Catalog Number: BYT-ORB101134-50,BYT-ORB101134-100,BYT-ORB101134-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human UGDH (421-494/494aa)
Conjugation: Unconjugated
Alternative Names: DEE84, EIEE84, GDH, UDP-GlcDH, UDPGDH, UGD, UGDH_HUMAN, UGDH, UDP-Glc dehydrogenase, 1.1.1.22, UGDH_MOUSE, UGDH_RAT,
UGDH Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 55 kDa
UniProt: O60701
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: UGDH
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (human descend colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (UGDH) Polyclonal Antibody, Unconjugated (orb101134) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (UGDH) Polyclonal Antibody, Unconjugated (orb101134) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (UGDH) Polyclonal Antibody, Unconjugated (orb101134) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (UGDH) Polyclonal Antibody, Unconjugated (orb101134) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Large intestine (Mouse) Lysate at 40 ug, Primary: Anti-UGDH (orb101134) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kD, Observed band size: 55 kD.
Sample: Liver (Mouse) Lysate at 40 ug, Stomach (Mouse) Lysate at 40 ug, Primary: Anti-UGDH (orb101134) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kD, Observed band size: 55 kD.