MOG Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB11068
- Bilder (6)
| Artikelname: | MOG Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB11068 |
| Hersteller Artikelnummer: | orb11068 |
| Alternativnummer: | BYT-ORB11068-100,BYT-ORB11068-200,BYT-ORB11068-50 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | WB |
| Spezies Reaktivität: | Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from mouse MOG (35-55/247aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | BTN6, BTNL11, MOGIG2, NRCLP7, B230317G11Rik, MOG_HUMAN, MOG, MOG_MOUSE, MOG_RAT, |
| MOG Rabbit Polyclonal Antibody |
| Application Verdünnung: | WB=1:500-2000 |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (MOG) Polyclonal Antibody, Unconjugated (orb11068) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (MOG) Polyclonal Antibody, Unconjugated (orb11068) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-Myelin-oligodendrocyte glycoprotein (orb11068) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 24 kD, Observed band size: 26 kD. |
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Sample: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-Myelin-oligodendrocyte glycoprotein (orb11068) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 24 kD, Observed band size: 26 kD. |
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Sample: Lane 1: Mouse Spinal cord tissue lysates, Lane 2: Rat Spinal cord tissue lysates, Primary: Anti-MOG (orb11068) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 24 kDa, Observed band size: 26 kDa. |
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Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-MOG Polyclonal Antibody, Unconjugated (orb11068) 1:200, overnight at 4⒉C, followed by conjugation to the secondary antibody and DAB staining. |
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