MOG Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB11068
Article Name: MOG Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB11068
Supplier Catalog Number: orb11068
Alternative Catalog Number: BYT-ORB11068-100,BYT-ORB11068-200,BYT-ORB11068-50
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: WB
Species Reactivity: Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from mouse MOG (35-55/247aa)
Conjugation: Unconjugated
Alternative Names: BTN6, BTNL11, MOGIG2, NRCLP7, B230317G11Rik, MOG_HUMAN, MOG, MOG_MOUSE, MOG_RAT,
MOG Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 24 kDa
Pubmed: 38227271
UniProt: Q61885
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: MOG
Application Dilute: WB=1:500-2000
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (MOG) Polyclonal Antibody, Unconjugated (orb11068) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (MOG) Polyclonal Antibody, Unconjugated (orb11068) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-Myelin-oligodendrocyte glycoprotein (orb11068) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 24 kD, Observed band size: 26 kD.
Sample: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-Myelin-oligodendrocyte glycoprotein (orb11068) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 24 kD, Observed band size: 26 kD.
Sample: Lane 1: Mouse Spinal cord tissue lysates, Lane 2: Rat Spinal cord tissue lysates, Primary: Anti-MOG (orb11068) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 24 kDa, Observed band size: 26 kDa.
Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-MOG Polyclonal Antibody, Unconjugated (orb11068) 1:200, overnight at 4⒉C, followed by conjugation to the secondary antibody and DAB staining.