|
Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Alpha-Synuclein) Polyclonal Antibody, Unconjugated (0968R) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Alpha-Synuclein) Polyclonal Antibody, Unconjugated (0968R) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
Paraformaldehyde-fixed, paraffin embedded (mouse spinal cord), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Alpha-Synuclein) Polyclonal Antibody, Unconjugated (0968R) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Alpha-Synuclein) Polyclonal Antibody, Unconjugated (0968R) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
Paraformaldehyde-fixed, paraffin embedded (rat spinal cord), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Alpha-Synuclein) Polyclonal Antibody, Unconjugated (0968R) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
Sample: B16 Cell (Mouse) Lysate at 30 ug, Primary: Anti-Alpha-Synuclein (orb11442) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 14 kD, Observed band size: 14 kD. |
|
Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Cerebellum tissue lysates, Lane 3: Mouse Hippocampus tissue lysates, Lane 4: Mouse Spinal cord tissue lysates, Lane 5: Rat Cerebrum tissue lysates, Lane 6: Rat Cerebellum tissue lysates, Lane 7: Rat Hippocampus tissue lysates, Primary: Anti-Alpha-Synuclein at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 14 kDa, Observed band size: 16 kDa. |