Alpha-Synuclein Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB11442
Article Name: Alpha-Synuclein Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB11442
Supplier Catalog Number: orb11442
Alternative Catalog Number: BYT-ORB11442-50,BYT-ORB11442-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant full length Alpha-Synuclein
Conjugation: Unconjugated
Alternative Names: NACP, PARK1, PARK4, PD1, alpha-Syn, alphaSYN, SYUA_HUMAN, SNCA, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor (NACP), SYUA_MOUSE, Syn, SYUA_RAT,
Alpha-Synuclein Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 14 kDa
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: SNCA
Application Dilute: WB=1:500-2000
Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Alpha-Synuclein) Polyclonal Antibody, Unconjugated (0968R) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Alpha-Synuclein) Polyclonal Antibody, Unconjugated (0968R) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse spinal cord), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Alpha-Synuclein) Polyclonal Antibody, Unconjugated (0968R) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Alpha-Synuclein) Polyclonal Antibody, Unconjugated (0968R) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat spinal cord), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Alpha-Synuclein) Polyclonal Antibody, Unconjugated (0968R) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: B16 Cell (Mouse) Lysate at 30 ug, Primary: Anti-Alpha-Synuclein (orb11442) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 14 kD, Observed band size: 14 kD.
Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Cerebellum tissue lysates, Lane 3: Mouse Hippocampus tissue lysates, Lane 4: Mouse Spinal cord tissue lysates, Lane 5: Rat Cerebrum tissue lysates, Lane 6: Rat Cerebellum tissue lysates, Lane 7: Rat Hippocampus tissue lysates, Primary: Anti-Alpha-Synuclein at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 14 kDa, Observed band size: 16 kDa.