Tyrosine Hydroxylase Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB11539
- Bilder (7)
| Artikelname: | Tyrosine Hydroxylase Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB11539 |
| Hersteller Artikelnummer: | orb11539 |
| Alternativnummer: | BYT-ORB11539-50,BYT-ORB11539-100,BYT-ORB11539-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from mouse TH (101-165/528aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | DYT14, DYT5b, TYH, The, TY3H_HUMAN, TH, Tyrosine 3-hydroxylase (TH), 1.14.16.2, TY3H_MOUSE, TY3H_RAT, |
| Tyrosine Hydroxylase Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 60 kDa |
| UniProt: | P24529 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | TH |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Tyrosine Hydroxylase) Polyclonal Antibody, Unconjugated (orb11539) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Tyrosine Hydroxylase) Polyclonal Antibody, Unconjugated (orb11539) at 1:400 overnight at 4C, followed by a conjugated secondary antibody (orb868805) for 90 minutes, and DAPI for nuclei staining. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Tyrosine Hydroxylase) Polyclonal Antibody, Unconjugated (orb11539) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Tyrosine Hydroxylase) Polyclonal Antibody, Unconjugated (orb11539) at 1:400 overnight at 4C, followed by a conjugated secondary antibody (orb868805) for 90 minutes, and DAPI for nuclei staining. |
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Sample: adipocyte (mouse) Lysate at 5-10 ug, model A, model B, model C are from different mice, Primary: Anti-Tyrosine Hydroxylase (orb11539) at 1/2000 dilution, Predicted band size: 60 kD, Observed band size: 60 kD. |
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Sample: PC-3 (Mouse) Lysate at 30 ug, Primary: Anti-Tyrosine Hydroxylase (orb11539) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000 dilution, Predicted band size: 60 kD, Observed band size: 60 kD. |
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Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Tyrosine Hydroxylase Polyclonal Antibody, Unconjugated (orb11539) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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