Tyrosine Hydroxylase Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB11539
- Images (7)
| Article Name: | Tyrosine Hydroxylase Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB11539 |
| Supplier Catalog Number: | orb11539 |
| Alternative Catalog Number: | BYT-ORB11539-50,BYT-ORB11539-100,BYT-ORB11539-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from mouse TH (101-165/528aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | DYT14, DYT5b, TYH, The, TY3H_HUMAN, TH, Tyrosine 3-hydroxylase (TH), 1.14.16.2, TY3H_MOUSE, TY3H_RAT, |
| Tyrosine Hydroxylase Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 60 kDa |
| UniProt: | P24529 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | TH |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
|
|
Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Tyrosine Hydroxylase) Polyclonal Antibody, Unconjugated (orb11539) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Tyrosine Hydroxylase) Polyclonal Antibody, Unconjugated (orb11539) at 1:400 overnight at 4C, followed by a conjugated secondary antibody (orb868805) for 90 minutes, and DAPI for nuclei staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Tyrosine Hydroxylase) Polyclonal Antibody, Unconjugated (orb11539) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Tyrosine Hydroxylase) Polyclonal Antibody, Unconjugated (orb11539) at 1:400 overnight at 4C, followed by a conjugated secondary antibody (orb868805) for 90 minutes, and DAPI for nuclei staining. |
|
|
Sample: adipocyte (mouse) Lysate at 5-10 ug, model A, model B, model C are from different mice, Primary: Anti-Tyrosine Hydroxylase (orb11539) at 1/2000 dilution, Predicted band size: 60 kD, Observed band size: 60 kD. |
|
|
Sample: PC-3 (Mouse) Lysate at 30 ug, Primary: Anti-Tyrosine Hydroxylase (orb11539) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000 dilution, Predicted band size: 60 kD, Observed band size: 60 kD. |
|
|
Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Tyrosine Hydroxylase Polyclonal Antibody, Unconjugated (orb11539) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
Product Guarantee and Expert Support
