H1F0 Antibody, Clone: [1415-1], Unconjugated, Mouse, Monoclonal

Artikelnummer: BYT-ORB1252534
Artikelname: H1F0 Antibody, Clone: [1415-1], Unconjugated, Mouse, Monoclonal
Artikelnummer: BYT-ORB1252534
Hersteller Artikelnummer: orb1252534
Alternativnummer: BYT-ORB1252534-100
Hersteller: Biorbyt
Wirt: Mouse
Kategorie: Antikörper
Applikation: FC, IF, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Nuclei of human leukemia biopsy cells were used as the immunogen for the Histone antibody.
Konjugation: Unconjugated
Alternative Synonym: H1F0, H10, H1FV, MGC5241
H1F0 Antibody
Klonalität: Monoclonal
Konzentration: 0.2 mg/mL
Klon-Bezeichnung: [1415-1]
UniProt: P07305
Puffer: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Formulierung: Liquid
Target-Kategorie: H1F0
Anwendungsbeschreibung: Application Notes: Western blot: 0.5-1 µg/mLFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 µg/mLImmunohistochemistry (FFPE): 0.5-1 µg/mL for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the Histone antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min
Formalin-fixed, paraffin-embedded human tonsil stained with Histone antibody (1415-1).
Formalin-fixed, paraffin-embedded human tonsil stained with Histone antibody (1415-1).
FFPE human testicular carcinoma tested with Histone H1 antibody (1415-1).
FFPE human pancreas tested with Histone H1 antibody (1415-1).
FFPE human ovarian carcinoma tested with Histone H1 antibody (1415-1).
FFPE human angiosarcoma tested with Histone H1 antibody (1415-1).
Western blot testing of HeLa cell lysate (nuclear fraction) with Histone antibody (clone 1415-1). Observed molecular weight ~22/30-33kDa (unmodified/modified).