PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Formulierung:
Liquid
Target-Kategorie:
H1F0
Anwendungsbeschreibung:
Application Notes: Western blot: 0.5-1 µg/mLFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 µg/mLImmunohistochemistry (FFPE): 0.5-1 µg/mL for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the Histone antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min
Formalin-fixed, paraffin-embedded human tonsil stained with Histone antibody (1415-1).
Formalin-fixed, paraffin-embedded human tonsil stained with Histone antibody (1415-1).
FFPE human testicular carcinoma tested with Histone H1 antibody (1415-1).
FFPE human pancreas tested with Histone H1 antibody (1415-1).
FFPE human ovarian carcinoma tested with Histone H1 antibody (1415-1).
FFPE human angiosarcoma tested with Histone H1 antibody (1415-1).
Western blot testing of HeLa cell lysate (nuclear fraction) with Histone antibody (clone 1415-1). Observed molecular weight ~22/30-33kDa (unmodified/modified).
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