H1F0 Antibody, Clone: [1415-1], Unconjugated, Mouse, Monoclonal

Catalog Number: BYT-ORB1252534
Article Name: H1F0 Antibody, Clone: [1415-1], Unconjugated, Mouse, Monoclonal
Biozol Catalog Number: BYT-ORB1252534
Supplier Catalog Number: orb1252534
Alternative Catalog Number: BYT-ORB1252534-100
Manufacturer: Biorbyt
Host: Mouse
Category: Antikörper
Application: FC, IF, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Nuclei of human leukemia biopsy cells were used as the immunogen for the Histone antibody.
Conjugation: Unconjugated
Alternative Names: H1F0, H10, H1FV, MGC5241
H1F0 Antibody
Clonality: Monoclonal
Concentration: 0.2 mg/mL
Clone Designation: [1415-1]
UniProt: P07305
Buffer: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Form: Liquid
Target: H1F0
Application Notes: Application Notes: Western blot: 0.5-1 µg/mLFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 µg/mLImmunohistochemistry (FFPE): 0.5-1 µg/mL for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the Histone antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min
Formalin-fixed, paraffin-embedded human tonsil stained with Histone antibody (1415-1).
Formalin-fixed, paraffin-embedded human tonsil stained with Histone antibody (1415-1).
FFPE human testicular carcinoma tested with Histone H1 antibody (1415-1).
FFPE human pancreas tested with Histone H1 antibody (1415-1).
FFPE human ovarian carcinoma tested with Histone H1 antibody (1415-1).
FFPE human angiosarcoma tested with Histone H1 antibody (1415-1).
Western blot testing of HeLa cell lysate (nuclear fraction) with Histone antibody (clone 1415-1). Observed molecular weight ~22/30-33kDa (unmodified/modified).