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IL25 Antibody, Unconjugated, Rabbit, Polyclonal

IL25 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1254260

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Artikelname:	IL25 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:	BYT-ORB1254260
Hersteller Artikelnummer:	orb1254260
Alternativnummer:	BYT-ORB1254260-100
Hersteller:	Biorbyt
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	IF, IHC, WB
Spezies Reaktivität:	Human, Mouse, Rat
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 33-177 of human IL25 (NP_073626.1).
Konjugation:	Unconjugated
Alternative Synonym:	IL25, interleukin 25, IL17E, interleukin 17E, interleukin-17E, interleukin-25

IL25 Antibody

Klonalität:	Polyclonal
Konzentration:	batch dependent
Molekulargewicht:	Observed: 24kDa
UniProt:	Q9H293
Puffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Formulierung:	Liquid
Target-Kategorie:	IL25

Anwendungsbeschreibung:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200

	Western blot analysis of extracts of various cell lines, using IL25 antibody (orb1254260) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded rat lung using IL25 antibody (orb1254260) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human prostate using IL25 antibody (orb1254260) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using IL25 antibody (orb1254260) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of human lung cancer cells using IL25 antibody (orb1254260) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of rat bronchus cells using IL25 antibody (orb1254260) at dilution of 1:100. Blue: DAPI for nuclear staining.

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