IL25 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1254260
Article Name: IL25 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1254260
Supplier Catalog Number: orb1254260
Alternative Catalog Number: BYT-ORB1254260-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 33-177 of human IL25 (NP_073626.1).
Conjugation: Unconjugated
Alternative Names: IL25, interleukin 25, IL17E, interleukin 17E, interleukin-17E, interleukin-25
IL25 Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 24kDa
UniProt: Q9H293
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: IL25
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using IL25 antibody (orb1254260) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat lung using IL25 antibody (orb1254260) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using IL25 antibody (orb1254260) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using IL25 antibody (orb1254260) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of human lung cancer cells using IL25 antibody (orb1254260) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat bronchus cells using IL25 antibody (orb1254260) at dilution of 1:100. Blue: DAPI for nuclear staining.
798,00 €
100 ul
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