NEFH Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1255836
Artikelname: NEFH Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: BYT-ORB1255836
Hersteller Artikelnummer: orb1255836
Alternativnummer: BYT-ORB1255836-100
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 821-1020 of human NEFH (NP_066554.2).
Konjugation: Unconjugated
Alternative Synonym: NEFH, 200 kDa neurofilament protein, KIAA0845, NFH, NF-H
NEFH Antibody
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 240kDa
UniProt: P12036
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Formulierung: Liquid
Target-Kategorie: NEFH
Anwendungsbeschreibung: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
Western blot analysis of extracts of mouse brain, using NEFH antibody (orb1255836) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
Immunohistochemistry of paraffin-embedded mouse kidney using NEFH antibody (orb1255836) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using NEFH antibody (orb1255836) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat cerebellum using NEFH antibody (orb1255836) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of mouse brain using NEFH antibody (orb1255836) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat brain using NEFH antibody (orb1255836) at dilution of 1:100. Blue: DAPI for nuclear staining.
798,00 €
100 ul
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