NEFH Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1255836
Article Name: NEFH Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1255836
Supplier Catalog Number: orb1255836
Alternative Catalog Number: BYT-ORB1255836-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 821-1020 of human NEFH (NP_066554.2).
Conjugation: Unconjugated
Alternative Names: NEFH, 200 kDa neurofilament protein, KIAA0845, NFH, NF-H
NEFH Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 240kDa
UniProt: P12036
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: NEFH
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
Western blot analysis of extracts of mouse brain, using NEFH antibody (orb1255836) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
Immunohistochemistry of paraffin-embedded mouse kidney using NEFH antibody (orb1255836) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using NEFH antibody (orb1255836) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat cerebellum using NEFH antibody (orb1255836) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of mouse brain using NEFH antibody (orb1255836) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat brain using NEFH antibody (orb1255836) at dilution of 1:100. Blue: DAPI for nuclear staining.