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CAD Antibody, Unconjugated, Rabbit, Polyclonal

CAD Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1255898

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Artikelname:	CAD Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:	BYT-ORB1255898
Hersteller Artikelnummer:	orb1255898
Alternativnummer:	BYT-ORB1255898-100
Hersteller:	Biorbyt
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	IF, IHC, WB
Spezies Reaktivität:	Human, Mouse
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1650-1900 of human CAD (NP_004332.2).
Konjugation:	Unconjugated
Alternative Synonym:	CAD protein, Glutamine-dependent carbamoyl-phosphate synthase, Aspartate carbamoyltransferase, Dihydroorotase, CAD

CAD Antibody

Klonalität:	Polyclonal
Konzentration:	batch dependent
Molekulargewicht:	Observed: 243kDa
UniProt:	P27708
Puffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Formulierung:	Liquid
Target-Kategorie:	CAD

Anwendungsbeschreibung:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200

	Western blot analysis of extracts of various cell lines, using CAD antibody (orb1255898) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human liver using CAD antibody (orb1255898) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using CAD antibody (orb1255898) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of NIH-3T3 cells using CAD antibody (orb1255898) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using CAD antibody (orb1255898) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using CAD antibody (orb1255898) at dilution of 1:100. Blue: DAPI for nuclear staining.

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