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CAD Antibody, Unconjugated, Rabbit, Polyclonal

CAD Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1255898

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Article Name:	CAD Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	BYT-ORB1255898
Supplier Catalog Number:	orb1255898
Alternative Catalog Number:	BYT-ORB1255898-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	IF, IHC, WB
Species Reactivity:	Human, Mouse
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1650-1900 of human CAD (NP_004332.2).
Conjugation:	Unconjugated
Alternative Names:	CAD protein, Glutamine-dependent carbamoyl-phosphate synthase, Aspartate carbamoyltransferase, Dihydroorotase, CAD

CAD Antibody

Clonality:	Polyclonal
Concentration:	batch dependent
Molecular Weight:	Observed: 243kDa
UniProt:	P27708
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form:	Liquid
Target:	CAD

Application Notes:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200

	Western blot analysis of extracts of various cell lines, using CAD antibody (orb1255898) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human liver using CAD antibody (orb1255898) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using CAD antibody (orb1255898) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of NIH-3T3 cells using CAD antibody (orb1255898) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using CAD antibody (orb1255898) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using CAD antibody (orb1255898) at dilution of 1:100. Blue: DAPI for nuclear staining.

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