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XRCC6 Antibody, Unconjugated, Rabbit, Polyclonal

XRCC6 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1256461

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Artikelname:	XRCC6 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:	BYT-ORB1256461
Hersteller Artikelnummer:	orb1256461
Alternativnummer:	BYT-ORB1256461-100
Hersteller:	Biorbyt
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	IF, IHC, IP, WB
Spezies Reaktivität:	Human, Monkey, Mouse, Rat
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 408-609 of human KU70 (NP_001460.1).
Konjugation:	Unconjugated
Alternative Synonym:	G22P1, ML8, KU70, TLAA, CTC75, CTCBF, G22P1

XRCC6 Antibody

Klonalität:	Polyclonal
Konzentration:	batch dependent
Molekulargewicht:	Observed: 70kDa
UniProt:	P12956
Puffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Formulierung:	Liquid
Target-Kategorie:	XRCC6

Anwendungsbeschreibung:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100IP: 1:20 - 1:50

	Western blot analysis of extracts of various cell lines, using KU70 antibody (orb1256461) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded rat brain using KU70 antibody (orb1256461) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using KU70 antibody (orb1256461) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human uterus using KU70 antibody (orb1256461) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using KU70 antibody (orb1256461). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 100 ug extracts of SW480 cells using 3 ug KU70 antibody (orb1256461). Western blot was performed from the immunoprecipitate using KU70 antibody (orb1256461) at a dilution of 1:1000.

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