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XRCC6 Antibody, Unconjugated, Rabbit, Polyclonal

XRCC6 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1256461

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Article Name:	XRCC6 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	BYT-ORB1256461
Supplier Catalog Number:	orb1256461
Alternative Catalog Number:	BYT-ORB1256461-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	IF, IHC, IP, WB
Species Reactivity:	Human, Monkey, Mouse, Rat
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 408-609 of human KU70 (NP_001460.1).
Conjugation:	Unconjugated
Alternative Names:	G22P1, ML8, KU70, TLAA, CTC75, CTCBF, G22P1

XRCC6 Antibody

Clonality:	Polyclonal
Concentration:	batch dependent
Molecular Weight:	Observed: 70kDa
UniProt:	P12956
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form:	Liquid
Target:	XRCC6

Application Notes:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100IP: 1:20 - 1:50

	Western blot analysis of extracts of various cell lines, using KU70 antibody (orb1256461) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded rat brain using KU70 antibody (orb1256461) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using KU70 antibody (orb1256461) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human uterus using KU70 antibody (orb1256461) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using KU70 antibody (orb1256461). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 100 ug extracts of SW480 cells using 3 ug KU70 antibody (orb1256461). Western blot was performed from the immunoprecipitate using KU70 antibody (orb1256461) at a dilution of 1:1000.

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