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BCAS3 Antibody, Unconjugated, Rabbit, Polyclonal

BCAS3 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1256493

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Artikelname:	BCAS3 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:	BYT-ORB1256493
Hersteller Artikelnummer:	orb1256493
Alternativnummer:	BYT-ORB1256493-100
Hersteller:	Biorbyt
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	IF, IHC, IP, WB
Spezies Reaktivität:	Human, Mouse, Rat
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 674-913 of human BCAS3 (NP_060149.3).
Konjugation:	Unconjugated
Alternative Synonym:	BCAS3 Antibody: MAAB, GAOB1, Breast carcinoma-amplified sequence 3

BCAS3 Antibody

Klonalität:	Polyclonal
Konzentration:	batch dependent
Molekulargewicht:	Observed: 115kDa
UniProt:	Q9H6U6
Puffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Formulierung:	Liquid
Target-Kategorie:	BCAS3

Anwendungsbeschreibung:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100

	Western blot analysis of extracts of various cell lines, using BCAS3 antibody (orb1256493) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human kidney cancer using BCAS3 antibody (orb1256493) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using BCAS3 antibody (orb1256493) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using BCAS3 antibody (orb1256493) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A-549 cells using BCAS3 antibody (orb1256493). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200 ug extracts of HeLa cells, using 3 ug BCAS3 antibody (orb1256493). Western blot was performed from the immunoprecipitate using BCAS3 antibody (orb1256493) at a dilution of 1:1000.

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