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BCAS3 Antibody, Unconjugated, Rabbit, Polyclonal

BCAS3 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1256493

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Article Name:	BCAS3 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	BYT-ORB1256493
Supplier Catalog Number:	orb1256493
Alternative Catalog Number:	BYT-ORB1256493-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	IF, IHC, IP, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 674-913 of human BCAS3 (NP_060149.3).
Conjugation:	Unconjugated
Alternative Names:	BCAS3 Antibody: MAAB, GAOB1, Breast carcinoma-amplified sequence 3

BCAS3 Antibody

Clonality:	Polyclonal
Concentration:	batch dependent
Molecular Weight:	Observed: 115kDa
UniProt:	Q9H6U6
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form:	Liquid
Target:	BCAS3

Application Notes:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100

	Western blot analysis of extracts of various cell lines, using BCAS3 antibody (orb1256493) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human kidney cancer using BCAS3 antibody (orb1256493) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using BCAS3 antibody (orb1256493) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using BCAS3 antibody (orb1256493) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A-549 cells using BCAS3 antibody (orb1256493). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200 ug extracts of HeLa cells, using 3 ug BCAS3 antibody (orb1256493). Western blot was performed from the immunoprecipitate using BCAS3 antibody (orb1256493) at a dilution of 1:1000.

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