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PNKP Antibody, Unconjugated, Rabbit, Polyclonal

PNKP Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1256844

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Artikelname:	PNKP Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:	BYT-ORB1256844
Hersteller Artikelnummer:	orb1256844
Alternativnummer:	BYT-ORB1256844-100
Hersteller:	Biorbyt
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	IF, IHC, WB
Spezies Reaktivität:	Human, Mouse, Rat
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 60-320 of human PNKP (NP_009185.2).
Konjugation:	Unconjugated
Alternative Synonym:	PNKP, polynucleotide kinase 3-phosphatase, PNK, polynucleotide kinase 3-prime-phosphatase, MCSZ, EIEE10

PNKP Antibody

Klonalität:	Polyclonal
Konzentration:	batch dependent
Molekulargewicht:	Observed: 57kDa
UniProt:	Q96T60
Puffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Formulierung:	Liquid
Target-Kategorie:	PNKP

Anwendungsbeschreibung:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100

	Western blot analysis of extracts of various cell lines, using PNKP antibody (orb1256844) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat kidney using PNKP antibody (orb1256844) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using PNKP antibody (orb1256844) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human prostate cancer using PNKP antibody (orb1256844) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse liver using PNKP antibody (orb1256844) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using PNKP antibody (orb1256844) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using PNKP antibody (orb1256844). Green:GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining. RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.

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