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PNKP Antibody, Unconjugated, Rabbit, Polyclonal

PNKP Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1256844

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Article Name:	PNKP Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	BYT-ORB1256844
Supplier Catalog Number:	orb1256844
Alternative Catalog Number:	BYT-ORB1256844-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	IF, IHC, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 60-320 of human PNKP (NP_009185.2).
Conjugation:	Unconjugated
Alternative Names:	PNKP, polynucleotide kinase 3-phosphatase, PNK, polynucleotide kinase 3-prime-phosphatase, MCSZ, EIEE10

PNKP Antibody

Clonality:	Polyclonal
Concentration:	batch dependent
Molecular Weight:	Observed: 57kDa
UniProt:	Q96T60
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form:	Liquid
Target:	PNKP

Application Notes:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100

	Western blot analysis of extracts of various cell lines, using PNKP antibody (orb1256844) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat kidney using PNKP antibody (orb1256844) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using PNKP antibody (orb1256844) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human prostate cancer using PNKP antibody (orb1256844) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse liver using PNKP antibody (orb1256844) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using PNKP antibody (orb1256844) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using PNKP antibody (orb1256844). Green:GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining. RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.

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