DDX1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1256922
Artikelname: DDX1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: BYT-ORB1256922
Hersteller Artikelnummer: orb1256922
Alternativnummer: BYT-ORB1256922-100
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, IP, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 441-740 of human DDX1 (NP_004930.1).
Konjugation: Unconjugated
Alternative Synonym: DDX1, DBP-RB, UKVH5d
DDX1 Antibody
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 100kDa
UniProt: Q92499
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Formulierung: Liquid
Target-Kategorie: DDX1
Anwendungsbeschreibung: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:10 - 1:100IP: 1:20 - 1:50
Western blot analysis of extracts of various cell lines, using DDX1 antibody (orb1256922) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat brain using DDX1 Antibody (orb1256922) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using DDX1 Antibody (orb1256922) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using DDX1 Antibody (orb1256922) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using DDX1 Antibody (orb1256922) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using DDX1 antibody (orb1256922). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 100 ug extracts of 293T cells using 3 ug DDX1 antibody (orb1256922). Western blot was performed from the immunoprecipitate using DDX1 antibody (orb1256922) at a dilution of 1:1000.