Western blot analysis of extracts of various cell lines, using DDX1 antibody (orb1256922) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat brain using DDX1 Antibody (orb1256922) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using DDX1 Antibody (orb1256922) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using DDX1 Antibody (orb1256922) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using DDX1 Antibody (orb1256922) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using DDX1 antibody (orb1256922). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 100 ug extracts of 293T cells using 3 ug DDX1 antibody (orb1256922). Western blot was performed from the immunoprecipitate using DDX1 antibody (orb1256922) at a dilution of 1:1000.
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