ATP5B Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1257339
Artikelname: ATP5B Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: BYT-ORB1257339
Hersteller Artikelnummer: orb1257339
Alternativnummer: BYT-ORB1257339-100
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 230-529 of human ATP5B (NP_001677.2).
Konjugation: Unconjugated
Alternative Synonym: ATP5B, ATPMB, ATPSB, MGC5231, HEL-S-271
ATP5B Antibody
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 57kDa
UniProt: P06576
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Formulierung: Liquid
Target-Kategorie: ATP5B
Anwendungsbeschreibung: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using ATP5B Antibody (orb1257339) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunohistochemistry of paraffin-embedded rat heart using ATP5B antibody (orb1257339) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using ATP5B antibody (orb1257339) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using ATP5B antibody (orb1257339) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using ATP5B antibody (orb1257339) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using ATP5B antibody (orb1257339) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using ATP5B antibody (orb1257339) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using ATP5B antibody (orb1257339) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.