ATP5B Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
BYT-ORB1257339
- Images (8)
| Article Name: | ATP5B Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | BYT-ORB1257339 |
| Supplier Catalog Number: | orb1257339 |
| Alternative Catalog Number: | BYT-ORB1257339-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 230-529 of human ATP5B (NP_001677.2). |
| Conjugation: | Unconjugated |
| Alternative Names: | ATP5B, ATPMB, ATPSB, MGC5231, HEL-S-271 |
| ATP5B Antibody |
| Clonality: | Polyclonal |
| Concentration: | batch dependent |
| Molecular Weight: | Observed: 57kDa |
| UniProt: | P06576 |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Form: | Liquid |
| Target: | ATP5B |
| Application Notes: | Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
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Western blot analysis of extracts of various cell lines, using ATP5B Antibody (orb1257339) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s. |
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Immunohistochemistry of paraffin-embedded rat heart using ATP5B antibody (orb1257339) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded human lung cancer using ATP5B antibody (orb1257339) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded human gastric cancer using ATP5B antibody (orb1257339) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded mouse heart using ATP5B antibody (orb1257339) at dilution of 1:100 (40x lens). |
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Immunofluorescence analysis of HeLa cells using ATP5B antibody (orb1257339) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of NIH/3T3 cells using ATP5B antibody (orb1257339) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of PC-12 cells using ATP5B antibody (orb1257339) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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