RFC4 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1257462
Artikelname: RFC4 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: BYT-ORB1257462
Hersteller Artikelnummer: orb1257462
Alternativnummer: BYT-ORB1257462-100
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, IP, WB
Spezies Reaktivität: Human, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 204-363 of human RFC4 (NP_002907.1).
Konjugation: Unconjugated
Alternative Synonym: RFC4, A1, MGC27291, RFC37
RFC4 Antibody
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 40kDa
UniProt: P35249
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Formulierung: Liquid
Target-Kategorie: RFC4
Anwendungsbeschreibung: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using RFC4 antibody (orb1257462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat testis using RFC4 Antibody (orb1257462) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using RFC4 Antibody (orb1257462) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using RFC4 Antibody (orb1257462) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using RFC4 antibody (orb1257462). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using RFC4 antibody (orb1257462). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of K562 cells using 1 ug RFC4 antibody (orb1257462). Western blot was performed from the immunoprecipitate using RFC4 antibody (orb1257462) at a dilution of 1:1000.