RFC4 Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
BYT-ORB1257462
- Images (7)
| Article Name: | RFC4 Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | BYT-ORB1257462 |
| Supplier Catalog Number: | orb1257462 |
| Alternative Catalog Number: | BYT-ORB1257462-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC, IP, WB |
| Species Reactivity: | Human, Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 204-363 of human RFC4 (NP_002907.1). |
| Conjugation: | Unconjugated |
| Alternative Names: | RFC4, A1, MGC27291, RFC37 |
| RFC4 Antibody |
| Clonality: | Polyclonal |
| Concentration: | batch dependent |
| Molecular Weight: | Observed: 40kDa |
| UniProt: | P35249 |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Form: | Liquid |
| Target: | RFC4 |
| Application Notes: | Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200 |
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Western blot analysis of extracts of various cell lines, using RFC4 antibody (orb1257462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. |
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Immunohistochemistry of paraffin-embedded rat testis using RFC4 Antibody (orb1257462) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded rat testis using RFC4 Antibody (orb1257462) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded human breast cancer using RFC4 Antibody (orb1257462) at dilution of 1:100 (40x lens). |
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Immunofluorescence analysis of U2OS cells using RFC4 antibody (orb1257462). Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of U2OS cells using RFC4 antibody (orb1257462). Blue: DAPI for nuclear staining. |
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Immunoprecipitation analysis of 200 ug extracts of K562 cells using 1 ug RFC4 antibody (orb1257462). Western blot was performed from the immunoprecipitate using RFC4 antibody (orb1257462) at a dilution of 1:1000. |
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