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PRKACB Antibody, Unconjugated, Rabbit, Polyclonal

PRKACB Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1257556

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Artikelname:	PRKACB Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:	BYT-ORB1257556
Hersteller Artikelnummer:	orb1257556
Alternativnummer:	BYT-ORB1257556-100
Hersteller:	Biorbyt
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	IF, IHC, WB
Spezies Reaktivität:	Human, Mouse, Rat
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-351 of human PRKACB (NP_002722.1).
Konjugation:	Unconjugated
Alternative Synonym:	PRKACB, Cbeta, PKACB, PKA C-beta, PKA Cbeta

PRKACB Antibody

Klonalität:	Polyclonal
Konzentration:	batch dependent
Molekulargewicht:	Observed: 37kDa
UniProt:	P22694
Puffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Formulierung:	Liquid
Target-Kategorie:	PRKACB

Anwendungsbeschreibung:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

	Western blot analysis of extracts of SW480 cells, using PRKACB antibody (orb1257556) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
	Western blot analysis of extracts of various cell lines, using PRKACB antibody (orb1257556) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded rat brain using PRKACB antibody (orb1257556) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human appendix using PRKACB antibody (orb1257556) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using PRKACB antibody (orb1257556). Blue: DAPI for nuclear staining.

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