PRKACB Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1257556
Article Name: PRKACB Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1257556
Supplier Catalog Number: orb1257556
Alternative Catalog Number: BYT-ORB1257556-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-351 of human PRKACB (NP_002722.1).
Conjugation: Unconjugated
Alternative Names: PRKACB, Cbeta, PKACB, PKA C-beta, PKA Cbeta
PRKACB Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 37kDa
UniProt: P22694
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: PRKACB
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of SW480 cells, using PRKACB antibody (orb1257556) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Western blot analysis of extracts of various cell lines, using PRKACB antibody (orb1257556) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat brain using PRKACB antibody (orb1257556) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human appendix using PRKACB antibody (orb1257556) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using PRKACB antibody (orb1257556). Blue: DAPI for nuclear staining.