Western blot analysis of extracts of SW480 cells, using PRKACB antibody (orb1257556) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Western blot analysis of extracts of various cell lines, using PRKACB antibody (orb1257556) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat brain using PRKACB antibody (orb1257556) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human appendix using PRKACB antibody (orb1257556) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using PRKACB antibody (orb1257556). Blue: DAPI for nuclear staining.
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