H3K14me2 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1257584
Artikelname: H3K14me2 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: BYT-ORB1257584
Hersteller Artikelnummer: orb1257584
Alternativnummer: BYT-ORB1257584-100
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ChIP, IF, IHC, IP, WB
Spezies Reaktivität: Mouse, Rat
Immunogen: A synthetic peptide of human DiMethyl-Histone H3-K14
Konjugation: Unconjugated
Alternative Synonym: H3F3A, H3t, H3.4, H3/g, H3FT
H3K14me2 Antibody
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 16kDa
UniProt: Q16695
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Formulierung: Liquid
Target-Kategorie: H3K14me2
Anwendungsbeschreibung: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100
Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K14 antibody (orb1257584). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K14 antibody (orb1257584) at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat liver using DiMethyl-Histone H3-K14 antibody (orb1257584) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human rectal cancer using DiMethyl-Histone H3-K14 antibody (orb1257584) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of C6 cells using DiMethyl-Histone H3-K14 pAb (orb1257584) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using DiMethyl-Histone H3-K14 pAb (orb1257584) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using DiMethyl-Histone H3-K14 pAb (orb1257584) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.