H3K14me2 Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
BYT-ORB1257584
- Images (7)
| Article Name: | H3K14me2 Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | BYT-ORB1257584 |
| Supplier Catalog Number: | orb1257584 |
| Alternative Catalog Number: | BYT-ORB1257584-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ChIP, IF, IHC, IP, WB |
| Species Reactivity: | Mouse, Rat |
| Immunogen: | A synthetic peptide of human DiMethyl-Histone H3-K14 |
| Conjugation: | Unconjugated |
| Alternative Names: | H3F3A, H3t, H3.4, H3/g, H3FT |
| H3K14me2 Antibody |
| Clonality: | Polyclonal |
| Concentration: | batch dependent |
| Molecular Weight: | Observed: 16kDa |
| UniProt: | Q16695 |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Form: | Liquid |
| Target: | H3K14me2 |
| Application Notes: | Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100 |
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Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K14 antibody (orb1257584). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
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Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K14 antibody (orb1257584) at 1:1000 dilution. |
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Immunohistochemistry of paraffin-embedded rat liver using DiMethyl-Histone H3-K14 antibody (orb1257584) at dilution of 1:200 (40x lens). |
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Immunohistochemistry of paraffin-embedded human rectal cancer using DiMethyl-Histone H3-K14 antibody (orb1257584) at dilution of 1:200 (40x lens). |
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Immunofluorescence analysis of C6 cells using DiMethyl-Histone H3-K14 pAb (orb1257584) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of NIH-3T3 cells using DiMethyl-Histone H3-K14 pAb (orb1257584) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of U-2 OS cells using DiMethyl-Histone H3-K14 pAb (orb1257584) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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