DUT Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1258016
Artikelname: DUT Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: BYT-ORB1258016
Hersteller Artikelnummer: orb1258016
Alternativnummer: BYT-ORB1258016-100
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 89-252 of human DUT (NP_001020419.1).
Konjugation: Unconjugated
Alternative Synonym: DUT, FLJ20622, dUTPase
DUT Antibody
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 24kDa
UniProt: P33316
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Formulierung: Liquid
Target-Kategorie: DUT
Anwendungsbeschreibung: Application Notes: WB: 1:500 - 1:1000IHC: 1:50 - 1:200IF: 1:100 - 1:200
Western blot analysis of extracts of various cell lines, using DUT antibody (orb1258016) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunohistochemistry of paraffin-embedded rat spleen using DUT antibody (orb1258016) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using DUT antibody (orb1258016) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human appendix using DUT antibody (orb1258016) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-2 OS cells using DUT Polyclonal Antibody (orb1258016) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.