DUT Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1258016
Article Name: DUT Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1258016
Supplier Catalog Number: orb1258016
Alternative Catalog Number: BYT-ORB1258016-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 89-252 of human DUT (NP_001020419.1).
Conjugation: Unconjugated
Alternative Names: DUT, FLJ20622, dUTPase
DUT Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 24kDa
UniProt: P33316
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: DUT
Application Notes: Application Notes: WB: 1:500 - 1:1000IHC: 1:50 - 1:200IF: 1:100 - 1:200
Western blot analysis of extracts of various cell lines, using DUT antibody (orb1258016) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunohistochemistry of paraffin-embedded rat spleen using DUT antibody (orb1258016) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using DUT antibody (orb1258016) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human appendix using DUT antibody (orb1258016) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-2 OS cells using DUT Polyclonal Antibody (orb1258016) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.