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VIM Antibody, Unconjugated, Rabbit, Polyclonal

VIM Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1258103

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Artikelname:	VIM Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:	BYT-ORB1258103
Hersteller Artikelnummer:	orb1258103
Alternativnummer:	BYT-ORB1258103-100
Hersteller:	Biorbyt
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	IF, IHC, WB
Spezies Reaktivität:	Human, Mouse
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human Vimentin (NP_003371.2).
Konjugation:	Unconjugated
Alternative Synonym:	Vimentin, VIM

VIM Antibody

Klonalität:	Polyclonal
Konzentration:	batch dependent
Molekulargewicht:	Observed: 55kDa
UniProt:	P08670
Puffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Formulierung:	Liquid
Target-Kategorie:	VIM

Anwendungsbeschreibung:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

	Western blot analysis of extracts of various cell lines, using Vimentin antibody (orb1258103) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human lung using Vimentin antibody (orb1258103) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using Vimentin antibody (orb1258103) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using Vimentin antibody (orb1258103) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using Vimentin antibody (orb1258103) at dilution of 1:100. Blue: DAPI for nuclear staining.

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