VIM Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1258103
Article Name: VIM Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1258103
Supplier Catalog Number: orb1258103
Alternative Catalog Number: BYT-ORB1258103-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human Vimentin (NP_003371.2).
Conjugation: Unconjugated
Alternative Names: Vimentin, VIM
VIM Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 55kDa
UniProt: P08670
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: VIM
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using Vimentin antibody (orb1258103) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human lung using Vimentin antibody (orb1258103) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using Vimentin antibody (orb1258103) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using Vimentin antibody (orb1258103) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using Vimentin antibody (orb1258103) at dilution of 1:100. Blue: DAPI for nuclear staining.
798,00 €
100 ul
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