H3K9me3 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1258188

Artikelname:	H3K9me3 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:	BYT-ORB1258188
Hersteller Artikelnummer:	orb1258188
Alternativnummer:	BYT-ORB1258188-100
Hersteller:	Biorbyt
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	ChIP, IF, IHC, IP, WB
Spezies Reaktivität:	Human, Mouse, Other, Rat
Immunogen:	A synthetic methylated peptide corresponding to residues surrounding K9 of human histone H3
Konjugation:	Unconjugated
Alternative Synonym:	H3F3A, H3t, H3.4, H3/g, H3FT

H3K9me3 Antibody

Klonalität:	Polyclonal
Konzentration:	batch dependent
Molekulargewicht:	Observed: 17kDa
UniProt:	Q16695
Puffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Formulierung:	Liquid
Target-Kategorie:	H3K9me3

Anwendungsbeschreibung:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:50 - 1:200CHIP seq: 1:50 - 1:200

	Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K9 antibody (orb1258188) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
	Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K9 antibody (orb1258188).
	Immunohistochemistry of paraffin-embedded rat testis using TriMethyl-Histone H3-K9 antibody (orb1258188) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using TriMethyl-Histone H3-K9 antibody (orb1258188) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of 293T cells using TriMethyl-Histone H3-K9 antibody (orb1258188). Blue: DAPI for nuclear staining.
	Chromatin immunoprecipitation analysis of extracts of 293T cells, using TriMethyl-Histone H3-K9 antibody (orb1258188) and rabbit IgG. P1 and P2 were located on EBAG9 gene. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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