H3K9me3 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1258188

Article Name:	H3K9me3 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	BYT-ORB1258188
Supplier Catalog Number:	orb1258188
Alternative Catalog Number:	BYT-ORB1258188-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	ChIP, IF, IHC, IP, WB
Species Reactivity:	Human, Mouse, Other, Rat
Immunogen:	A synthetic methylated peptide corresponding to residues surrounding K9 of human histone H3
Conjugation:	Unconjugated
Alternative Names:	H3F3A, H3t, H3.4, H3/g, H3FT

H3K9me3 Antibody

Clonality:	Polyclonal
Concentration:	batch dependent
Molecular Weight:	Observed: 17kDa
UniProt:	Q16695
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form:	Liquid
Target:	H3K9me3

Application Notes:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:50 - 1:200CHIP seq: 1:50 - 1:200

	Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K9 antibody (orb1258188) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
	Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K9 antibody (orb1258188).
	Immunohistochemistry of paraffin-embedded rat testis using TriMethyl-Histone H3-K9 antibody (orb1258188) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using TriMethyl-Histone H3-K9 antibody (orb1258188) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of 293T cells using TriMethyl-Histone H3-K9 antibody (orb1258188). Blue: DAPI for nuclear staining.
	Chromatin immunoprecipitation analysis of extracts of 293T cells, using TriMethyl-Histone H3-K9 antibody (orb1258188) and rabbit IgG. P1 and P2 were located on EBAG9 gene. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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