Western blot analysis of extracts of various cell lines, using Histone H3 antibody (orb1258194) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat kidney using Histone H3 antibody (orb1258194) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using Histone H3 antibody (orb1258194) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using Histone H3 antibody (orb1258194) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 150 ug extracts of MCF7 cells using 3 ug Histone H3 antibody (orb1258194). Western blot was performed from the immunoprecipitate using Histone H3 antibody (orb1258194) at a dilution of 1:500.
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