HIST3H3 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1258194
Article Name: HIST3H3 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1258194
Supplier Catalog Number: orb1258194
Alternative Catalog Number: BYT-ORB1258194-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IHC, IP, WB
Species Reactivity: Human, Mouse, Other, Rat
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human HIST3H3 (NP_003484.1).
Conjugation: Unconjugated
Alternative Names: H3F3A, H3t, H3.4, H3/g, H3FT
HIST3H3 Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 17kDa
UniProt: Q16695
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: HIST3H3
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:20 - 1:50
Western blot analysis of extracts of various cell lines, using Histone H3 antibody (orb1258194) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat kidney using Histone H3 antibody (orb1258194) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using Histone H3 antibody (orb1258194) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using Histone H3 antibody (orb1258194) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 150 ug extracts of MCF7 cells using 3 ug Histone H3 antibody (orb1258194). Western blot was performed from the immunoprecipitate using Histone H3 antibody (orb1258194) at a dilution of 1:500.
798,00 €
100 ul
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