Western blot analysis of extracts of various cell lines, using ERK1/ERK2 antibody (orb1258906) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunofluorescence analysis of C6 cells using ERK1/ERK2 antibody (orb1258906) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using ERK1/ERK2 antibody (orb1258906) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using ERK1/ERK2 antibody (orb1258906) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using ERK1/ERK2 Polyclonal Antibody (orb1258906) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using ERK1/ERK2 Polyclonal Antibody (orb1258906) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using ERK1/ERK2 Polyclonal Antibody (orb1258906) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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