MAPK1/MAPK3 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1258906
Artikelname: MAPK1/MAPK3 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: BYT-ORB1258906
Hersteller Artikelnummer: orb1258906
Alternativnummer: BYT-ORB1258906-100
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant Protein of human ERK1 / ERK2
Konjugation: Unconjugated
Alternative Synonym: ERK, ERT1, MAPK 1, MAPK2, ERK2
MAPK1/MAPK3 Antibody
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 42kDa/44kDa
UniProt: P27361
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Formulierung: Liquid
Target-Kategorie: MAPK1/MAPK3
Anwendungsbeschreibung: Application Notes: WB: 1:1000 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using ERK1/ERK2 antibody (orb1258906) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunofluorescence analysis of C6 cells using ERK1/ERK2 antibody (orb1258906) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using ERK1/ERK2 antibody (orb1258906) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using ERK1/ERK2 antibody (orb1258906) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using ERK1/ERK2 Polyclonal Antibody (orb1258906) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using ERK1/ERK2 Polyclonal Antibody (orb1258906) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using ERK1/ERK2 Polyclonal Antibody (orb1258906) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.