MAPK1/MAPK3 Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
BYT-ORB1258906
- Images (7)
| Article Name: | MAPK1/MAPK3 Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | BYT-ORB1258906 |
| Supplier Catalog Number: | orb1258906 |
| Alternative Catalog Number: | BYT-ORB1258906-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Recombinant Protein of human ERK1 / ERK2 |
| Conjugation: | Unconjugated |
| Alternative Names: | ERK, ERT1, MAPK 1, MAPK2, ERK2 |
| MAPK1/MAPK3 Antibody |
| Clonality: | Polyclonal |
| Concentration: | batch dependent |
| Molecular Weight: | Observed: 42kDa/44kDa |
| UniProt: | P27361 |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Form: | Liquid |
| Target: | MAPK1/MAPK3 |
| Application Notes: | Application Notes: WB: 1:1000 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
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Western blot analysis of extracts of various cell lines, using ERK1/ERK2 antibody (orb1258906) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. |
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Immunofluorescence analysis of C6 cells using ERK1/ERK2 antibody (orb1258906) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of NIH/3T3 cells using ERK1/ERK2 antibody (orb1258906) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of U2OS cells using ERK1/ERK2 antibody (orb1258906) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of C6 cells using ERK1/ERK2 Polyclonal Antibody (orb1258906) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of NIH-3T3 cells using ERK1/ERK2 Polyclonal Antibody (orb1258906) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of U-2 OS cells using ERK1/ERK2 Polyclonal Antibody (orb1258906) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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