IL2 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1259059
Artikelname: IL2 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: BYT-ORB1259059
Hersteller Artikelnummer: orb1259059
Alternativnummer: BYT-ORB1259059-100
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant protein of human IL2
Konjugation: Unconjugated
Alternative Synonym: Interleukin 2Interleukin-2, T-cell growth factor, IL-2, TCGF, lymphokine
IL2 Antibody
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 18kDa
UniProt: P60568
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Formulierung: Liquid
Target-Kategorie: IL2
Anwendungsbeschreibung: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using IL2 antibody (orb1259059) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Western blot analysis of extracts of Raw264.7 cells, using IL2 antibody (orb1259059) at 1:1000 dilution. Raw264.7 cells were treated by LPS for 6 hours and Brefeldin A for 3 hours of stimulation. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human tonsil using IL2 antibody (orb1259059) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using IL2 antibody (orb1259059) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using IL2 antibody (orb1259059) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using IL2 antibody (orb1259059) at dilution of 1:100 (40x lens).