Western blot analysis of extracts of various cell lines, using IL2 antibody (orb1259059) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Western blot analysis of extracts of Raw264.7 cells, using IL2 antibody (orb1259059) at 1:1000 dilution. Raw264.7 cells were treated by LPS for 6 hours and Brefeldin A for 3 hours of stimulation. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human tonsil using IL2 antibody (orb1259059) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using IL2 antibody (orb1259059) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using IL2 antibody (orb1259059) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using IL2 antibody (orb1259059) at dilution of 1:100 (40x lens).
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