Western blot analysis of extracts of various cell lines, using FBL antibody (orb1260398) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse liver using FBL antibody (orb1260398) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using FBL antibody (orb1260398) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using FBL antibody (orb1260398) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using FBL antibody (orb1260398) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using FBL antibody (orb1260398) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of HeLa cells using 1 ug FBL antibody (orb1260398). Western blot was performed from the immunoprecipitate using FBL antibody (orb1260398) at a dilution of 1:1000.
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