FBL Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1260398
Article Name: FBL Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1260398
Supplier Catalog Number: orb1260398
Alternative Catalog Number: BYT-ORB1260398-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, IP, WB
Species Reactivity: Human, Monkey, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 100-321 of human FBL (NP_001427.2).
Conjugation: Unconjugated
Alternative Names: FBL, FIB, FLRN, RNU3IP1
FBL Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 37kDa
UniProt: P22087
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: FBL
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50
Western blot analysis of extracts of various cell lines, using FBL antibody (orb1260398) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse liver using FBL antibody (orb1260398) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using FBL antibody (orb1260398) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using FBL antibody (orb1260398) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using FBL antibody (orb1260398) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using FBL antibody (orb1260398) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of HeLa cells using 1 ug FBL antibody (orb1260398). Western blot was performed from the immunoprecipitate using FBL antibody (orb1260398) at a dilution of 1:1000.